The assay begins with a standard kinase reaction performed in the provided reaction Buffer with a provided Bisamide Rhodamine 110 peptide substrate (PKA R110 substrate).

检测开始时、先用试剂盒提供的PKA双酰胺罗丹明110肽底物进行一个标准的激酶反应。

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The acidity for hydride generation reaction, selection of buffer solution and oxidizer, dynamic linear range, interference from coexistent elements and their elimination were studied.

主要对锗、锡的氢化反应酸度，缓冲溶液的选择，动态线性范围，氧化剂的选择，共存元素的干扰及其消除进行了实验。

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For partners and spouses, "it is vital that they learn to create a buffer between the person's reaction to stress and their own experience, " says Robert Lawrence Friedman, president of Stress Solutions, a Forest Hills, N.

WSJ: When Your Partner Overreacts to Stress